









WARNING: THIS TEST DOES NOT EVALUATE DRUG SAFETY OR PURITY
The Rapid Response™ Nitazene Test Strip (Liquid/Powder) is a rapid visual immunoassay for the qualitative, presumptive detection of Nitazene in suspicious substances (liquids or powders) at the cut-off concentration of 2000 ng/mL. For forensic use only. Not an IVD.
A recent study from the Laboratory of Toxicology, Ghent University showed BTNX's Rapid Response™ Nitazene Test Strips are capable of detecting the presence of a wide range of nitazene analogues and may be used to alert users of the presence of nitazene analogues in authentic (street) drug samples. Read the study here.
WARNING: THIS TEST DOES NOT EVALUATE DRUG SAFETY OR PURITY
The Rapid Response™ Nitazene Test Strip (Liquid/Powder) is a rapid visual immunoassay for the qualitative, presumptive detection of Nitazene in suspicious substances (liquids or powders) at the cut-off concentration of 2000 ng/mL. For forensic use only. Not an IVD.
A recent study from the Laboratory of Toxicology, Ghent University showed BTNX's Rapid Response™ Nitazene Test Strips are capable of detecting the presence of a wide range of nitazene analogues and may be used to alert users of the presence of nitazene analogues in authentic (street) drug samples. Read the study here.
Easy to use with detailed instructions
Individually packed strips for easy distribution
Detects Nitazene in concentrations at and above 2000 ng/mL
Instant Results*
For Liquid or Powder samples
Results interpretation printed on every pouch
Nitazenes are a class of synthetic drugs that have gained attention in the United States and other countries in recent years. The prevalence of nitazenes in the illegal drug supply has been on the rise since 2019. Recent evidence suggests nitazenes may be more potent than fentanyl and may require a higher dose of naloxone in case of overdose.1 In the same study, several individuals who overdosed on nitazenes were unaware that their drugs contained it.1
Reference: 1. JAMA Netw Open. 2023;6(8):e2331264.doi:10.1001/jamanetworkopen.2023.31264
- Enhanced User Guidance: Every pouch features a QR code linking it to the BTNX Harm Reduction Resource Page - giving every user access to written and video instructions.
- Straightforward Results Interpretation: Guide printed on the back of every pouch and T’s and C’s printed on each strip to indicate the orientation of the Test and Control lines.
- Compact Design: Individually packaged strips facilitate easy distribution.
- Quick Results: Obtain results as soon as 1 minute.*
- Procedural Control: Every strip features a built-in procedural control mechanism.
The following tables list the concentrations of compounds (ng/mL) above which the Rapid Response™ Nitazene Test Strip (Liquid / Powder) identified positive.
Nitazene related compounds |
Concentration (ng/ml) |
Isotonitazene |
2000 |
Protonitazene |
3000 |
N-Pyrrolidono Etonitazene |
2000 |

Please refer to the product insert for more details.

Public Health Ontario's review highlights the positive impact of drug checking test strips, indicating potential for overdose reduction and safer drug use. Read the study here Public Health Ontario Rapid Review.
Nitazenes are a class of synthetic drugs that have gained attention in the United States and other countries in recent years. The prevalence of nitazenes in the illegal drug supply has been on the rise since 2019. Nitazenes are ultra-high potency synthetic opioids, Isotonitazene and Protonitazene are up to 8 times stronger than fentanyl, while etonitazepyne and protonitazepyne surpass fentanyl’s potency by a staggering 20 times.
Toronto Drug Checking services have detected various types of Nitazenes in anticipated Fentanyl drug samples, alongside other substances. The breakdown of percentages is as follows: Metonitazene at 5%, Isotonitazene/Protonitazene at 2%, and N-desethyl isotonitazene, along with other variants, accounting for less than 1%2.
In the UK, nitazenes have been detected in substances sold as other opioids such as heroin and oxycodone; benzodiazepines, including diazepam; and cannabis products.3
DEA regional forensic laboratories have seen Nitazenes mixed into heroin and/or fentanyl.4
Recent evidence suggests nitazenes may be more potent than fentanyl and may require a higher dose of naloxone in case of overdose.1 In the same study several individuals who overdosed on nitazenes were unaware that their drugs contained it.1
- JAMA Netw Open. 2023;6(8):e2331264. doi:10.1001/jamanetworkopen.2023.31264
- Toronto Drug Checking Services: Expected Fentanyl samples containing other substances.
- Responding To Synthetic Opioids In The UK Drugs Market. Transform Drug Policy Foundation. https://transformdrugs.org/blog/responding-to-nitazenes-in-the-uk-drugs-market#:~:text=Nitazenes%20are%20synthetic%20opioids%20%2D%20laboratory,like%20diazepam%3B%20and%20cannabis%20products. December 1, 2023. Accessed April 19, 2024.
- New, Dangerous Synthetic Opioid in D.C., Emerging in Tri-State Area. DEA Washington, DC Division – Public Information Office. DEA United States Drug Enforcement Administration. https://www.dea.gov/stories/2022/2022-06/2022-06-01/new-dangerous-synthetic-opioid-dc-emerging-tri-state-area. June 1, 2022. Accessed April 19, 2024.
*A negative result can be interpreted as soon as both the test (T) and control (C) lines appear. A positive result can be interpreted once the control line has appeared and the background has cleared to white, typically by 60 seconds. Do not read results after 10 minutes.
- The test device is NOT intended to determine the purity, composition, or if the substance being examined is safe to use.
- A positive or negative test result is NOT an indication that the substance being examined is safe to use. Many factors come into play when examining the samples, including but not limited to mixture of multiple substances, solubility, and pH of the sample.
- BTNX Inc. does not encourage the use, supply, or production of illegal drugs or controlled substances in any way. The device is intended for harm reduction purposes. Follow the advice of your local harm reduction or public health agency.
- There is a possibility that technical or procedural errors as well as other substances and factors may interfere with the Rapid Response™ Nitazene Test Strip (Liquid / Powder) and cause false results.
- A positive result indicates the presence of nitazene only and does not indicate quantity.
- A negative result does not at any time rule out the presence of nitazene, as they may be present below the minimum detection level of the test.
- Please note that certain products may only be available in specific regions; kindly consult with a sales representative for further information regarding product availability.
- The information provided on this website is for educational purposes only and should not be construed as medical advice. Always consult with a qualified healthcare professional regarding any medical concerns or conditions.
- Our products are intended for use as specified in the product documentation. It is important to carefully read and follow all instructions provided with the product.